Direct measurement of protein osmotic second virial cross coefficients by cross-interaction chromatography
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چکیده
منابع مشابه
Negative second virial coefficients as predictors of protein crystal growth: evidence from sedimentation equilibrium studies that refutes the designation of those light scattering parameters as osmotic virial coefficients.
The effects of ammonium sulphate concentration on the osmotic second virial coefficient (BAA/MA) for equine serum albumin (pH 5.6, 20 degrees C) have been examined by sedimentation equilibrium. After an initial steep decrease with increasing ammonium sulphate concentration, BAA/MA assumes an essentially concentration-independent magnitude of 8-9 ml/g. Such behaviour conforms with the statistica...
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A short self-contained derivation is given for the second virial coefficient B2(T) of a gas consisting of identical interacting asymmetric rigid rotors. The resulting expression is correct through variant Planck's over h2. First, the canonical partition function is derived by means of an variant Planck's over h expansion of exp[-H/(k(B)T)] due to Friedmann [Adv. Chem. Phys. 4, 225 (1962)]. The ...
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Important progress has been made in recent years toward developing a molecular-level understanding of protein phase behavior in terms of the osmotic second virial coefficient, a thermodynamic parameter that characterizes pairwise protein interactions. Yet there has been little practical application of this knowledge to the field of protein crystallization, largely because of the difficult and t...
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ژورنال
عنوان ژورنال: Protein Science
سال: 2004
ISSN: 0961-8368,1469-896X
DOI: 10.1110/ps.03419204